Laboratory of Neural Network Function School of Brain and Cognitive Sciences Beijing Normal University 19 Xinjiekou Outer Street, Haidian District, Beijing 100875 P.R. China Lab Head: Yousheng Shu Email: email@example.com Links: National Key Laboratory of Cognitive Neuroscience and Learning
- Welcome to Yousheng Shu Lab
We study the axon physiology, the role of axonal ion channels and neurotransmitter receptors in regulating neuronal signaling, and the generation of network activity at different behavior states. In addition, we investigate the changes of channel property, synaptic strength and network activity pattern in diseased brain, and seek for efficient treatment for brain disorders, such as epilepsy, depression,and mental retardation. For more...
- Selected publications:
We performed dual whole-cell recordings from pyramidal cells (PCs) and nearby inhibitory interneurons in layer 5 of rodent neocortical slices. We found asynchronous release (AR) of glutamate occurs at PC output synapses onto Martinotti cells (MCs), causing desynchronized and prolonged firing in MCs and thus imprecise and long-lasting inhibition in neighboring PCs. These results highlight the effect of glutamate AR on the operation of microcircuits mediating slow recurrent inhibition, an important mechanism for controlling the timing and size of cortical inhibition. Deng et al., 2020 .
|We performed 2-photon axotomy during simultaneous soma-axon recordings to isolate autaptic responses in neocortical pyramidal cells (PCs). We found that autapses (self-synapses) selectively form in subcortically projecting PCs and promote their burst firing by producing giant AMPA-only aEPSCs. Yin, Zheng, Ke, He et al., 2018 .|
|Activation of 5-HT1A┬áreceptors selectively inhibits┬áNa+┬áchannel subtype Nav1.2 but not Nav1.6, thus┬ácould decrease the success rate of action potential backpropagation toward the somatodendritic compartments, enhancing the┬ásegregation of axonal and dendritic activities. Yin et al., 2015 .┬á|
Our whole-cell recordings from human and monkey cortical slices reveal a distinct subpopulation of inhibitory interneuron with intrinsic persistent activity. Weak stimuli can turn on┬áthe persistent activity; however,┬ástrong stimuli can turn it off. This type of neuron could not be found in┬árodent cortical tissues, suggesting it could be unique to primates. This study is a good start to characterize cell types in human tissue with distinct physiological properties. Wang et al., 2015 .┬á
We performed patch-clamp recording from axon blebs of cortical inhibitory interneurons (PV and SST cells).┬á┬áDifferences in voltage dependence of their┬áAIS Na+ channels result from distinct distribution patterns of channel subtypes. Surprisingly, Nav1.2 is expressed by SST cells. Li et al., 2014 .
|Immunostaining of Na+ channel subtypes in human cortical tissue. We found segregated distribution of proximal-Nav1.2 and distal-Nav1.6 at PC AIS. In addition to Nav1.6, the nodes of Ranvier in adult human cortex also express Nav1.2. We found no immunosignal of Nav1.1 at PC AIS. Tian et al.,2014 .|
- We found that Na+ channel subtype Nav1.6 determines action potential initiation and Nav1.2 promotes its backpropagation. Hu et al., 2009 . Also see comments: Dulla and Huguenard, 2009 .
Membrane potential-dependent regulation of cortical recurrent inhibition. Zhu et al., 2011 . Also see .
- Asynchronous GABA release is upregulated in epileptic cortical tissue. Jiang et al., 2012 .